gentleMACSâ„¢ Dissociator
Description The gentleMACS™ Dissociator is a benchtop instrument for the automated dissociation of tissues. Two types of unique gentleMACS Tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system. C Tubes are used for the gentle preparation of single-cell suspensions from tissues, whereas M Tubes are used for the homogenization of tissues or cells. A single sample or two samples in parallel can be processed. The instrument offers optimized gentleMACS Programs for a variety of specific applications. The standardized tissue dissociation or homogenization procedures make for reliable and reproducible results. The gentleMACS Dissociator: Time-saving automated tissue dissociation Standardized procedure High level of user safety Sterile sample handling Applications The gentleMACS Dissociator in combination with C Tubes is designed for the preparation of single-cell suspensions from various tissues. The tissue dissociation procedure is gentle to cells and yields cell suspensions with a high viability rate. Specific gentleMACS Programs and Protocols are available for the dissociation of various tissues. Dissociation of mouse spleen Dissociation of mouse spleen using Collagenase D Dissociation of mouse lung using Collagenase D Dissociation of mouse liver using Collagenase D Dissociation of mouse brain in combination with MACS® Neural Tissue Dissociation Kits Homogenization of tissues to extract biomolecules for molecular biology experiments is performed using M Tubes. gentleMACS Programs and Protocols for the fast and thorough tissue homogenization are available for the isolation of total RNA or mRNA. Miltenyi Biotec continuously expands the range of specific applications for the gentleMACS Dissociator. Watch out for new gentleMACS Programs and Protocols. Further information Preparation of single-cell suspensions from mouse spleen [PDF; 54,9 KB] Preparation of single-cell suspensions from mouse spleen with Collagenase D treatment [PDF; 83,2 KB] Preparation of single-cell suspensions from mouse liver with Collagenase D treatment [PDF; 65,2 KB] Preparation of single-cell suspensions from mouse neural tissue [PDF; 114,6 KB] Preparation of single-cell suspensions from mouse lung with Collagenase D treatment [PDF; 78,2 KB] Homogenization of tissue for total RNA isolation [PDF; 71,7 KB] Homogenization of tissue for mRNA isolation [PDF; 72,1 KB] gentleMACS Dissociator user manual, version 3 [PDF; 777,9 KB] gentleMACS Protocol overview [PDF; 69,6 KB] gentleMACS Dissociator flyer [PDF; 83,3 KB]		Figure 1 gentleMACS NEWS New protocol: dissociation of mouse lung Sign in for demo Upgrade your instrument with the new programs m_lung_01 and m_lung_02 Figure 2 The gentleMACS™ Dissociator, shown with M Tubes and C Tubes Figure 3 Introductory video Watch the short educational video to learn how the gentleMACS Dissociator can make your work easier.

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